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Procedure (a) Remove medium from a ask containing MEFs and wash cells once with 5 mL of PBSA per 75-cm2 ask. (b) Add 1 mL of trypsin per 75-cm2 ask, ensuring that the whole monolayer is covered, and incubate at 37 C for 4 min. (c) Gently tap the ask to further dislodge the cells. Neutralize the trypsin with 5 mL of MEFM and triturate 5 6 times to ensure cells are dislodged and to prevent clumping. Transfer medium containing MEFs to a 15-mL centrifuge tube. (d) Centrifuge at 1000 g for 5 min. (e) Label and prepare cryovials for freezing. Labeling should include the new passage number of MEFs, the date, and any other information that may be needed. Prepare cryovials for freezing by adding 100 L of DMSO in sterile conditions. (f) Aspirate the supernate from the cells, taking care not to disturb the pellet. (g) Resuspend the pellet in 900 L of ESFBS per 75-cm2 ask, making sure the pellet is evenly mixed by triturating with a plastic pipette. (h) Transfer the ESFBS containing the MEFs to the cryovial containing DMSO and mix thoroughly before immediately storing at 80 C overnight. (i) The next day, move the cryovial into liquid nitrogen for long-term storage.

Functions are usually defined at the very beginning of a Flash movie (or within a Movie Clip instance that loads within the first frames of Flash movie). You should only place functions on timeline keyframes you can, however, execute functions from any event handler in Flash.

var ctPhotograph:ColorTransform = new ColorTransform(); ctPhotograph.redOffset = 255; mPhotograph.transform.colorTransform = ctPhotograph;

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The user agent MUST perform the following steps to execute a refresh task: 1. For each wml:setvar element, the variable name and value in each wml:setvar element is converted into a simple string by substituting all referenced variables. See section 5.5.1.1 for more information on variable substitution. 2. The variable assignments resulting from the processing done in step #1 (the wml:setvar element) are applied to the current user agent context. 3. If the destination contains any locally scoped form data, it is refreshed with the form data stored in the entry at the top of the history stack. 4. If the current body/card contains a wml:timer element, the timer is started as speci ed in section 6.16.11. 5. The current body/card is re-displayed using the current variable state and processing stops. User-visible effects of the state changes (e.g., a change in the screen display resulting from the changes of referenced variables) SHALL occur during the processing of the refresh task. A refresh and its user-visible effects SHALL occur even if the elements have no wml:setvar elements, given that context may change by other means (e.g., timer).

Figure 20.9 shows the resulting application, with each contact s full name in the left column and complete formatted address information in the right column. The user can select the text in the right column and then right-click (or Ctrl+click on the Mac) to copy the text with the pop-up context menu.

Figure 11-21: The vector object on the bottom left is filled completely by the Paint Bucket. Applying the same fill to the bitmap object on the bottom right only affects pixels colored similarly to those under the cursor.

With HTML version 4.01, most presentation attributes of the <body> tag have been deprecated in favor of specifying these attributes as styles. In previous versions of HTML, you could specify a bevy of options, including the document background, text, and link colors. The <body> tag s onload and onunload attributes, as well as global attributes such as style, are still valid. However, you should specify the other attributes in styles instead, as in the following example:

where the hook numbers are displayed in the denominator boxes. For SU(2) this corresponds to the familiar result 2 @2 = 3@ 1, while for S U ( 3 ) (see also Exercise 5 . 1 2 ~ ) (5.85)

6

A loan amortization schedule is a table of values that shows various pieces of information for each payment period of a loan. Figure 16.5 shows a worksheet that uses formulas to calculate an amortization schedule.

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