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The latter is the capability of the method to discriminate small differences in concentration or mass of the test analyte and is equal to the slope of the calibration curve (see below) [56] Limit of Quanti cation For the limit of quanti cation, or limit of determination, de nitions and formulas are very similar to those of LOD, except that for LOQ, k is taken to be 5, 6, or even 10 [2, 4, 15, 56, 72, 96] A value of 10 for k means that the %RSD at the limit of quanti cation is 10% The LOQ thus corresponds to that concentration or amount of analyte quanti able with a variation coef cient not higher than 10% [98] The LOQ is always higher than the LOD and is often taken as a xed multiple (typically 2) of the detection limit [4].

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Also, the determination limit is referred to as the signal 10 times above the noise or background signal, corresponding to a signal-to-noise ratio of 10 : 1 [72, 85] In practice, the LOQ can be calculated in an analogous way as for the LOD, as indicated in Table 5 An alternative way of practically assessing the LOD and LOQ is the following In a rst step, 10 independent sample blanks are measured each once, the blank standard deviation sbl is calculated, and the lowest signals corresponding to both the LOD and the LOQ are calculated as xLOD = xbl + 3sbl and xLOQ = xbl + 10sbl, respectively In a second step, sample blanks are spiked with various analyte concentrations (eg, 6) close to the LOD Per concentration, 10 independent replicates are measured and the standard deviation of the measured signals calculated.

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one medium, such as a soft-copy display, and the reproduction is viewed in a second medium, such as a hard-copy output A series of experiments have been described (Fairchild 1992b, 1993a) that quantify some of the characteristics of chromatic-adaptation mechanisms and indicate that the same mechanisms are not active when soft-copy displays are viewed as are active when hard-copy displays or original scenes are viewed. When hard-copy images are being viewed, an image is perceived as an object that is illuminated by the prevailing illumination. Thus both sensory mechanisms, that respond to the spectral energy distribution of the stimulus, and cognitive mechanisms, that discount the known color of the light source, are active. When a soft-copy display is being viewed, it cannot easily be interpreted as an illuminated object. Therefore there is no known illuminant color and only sensory mechanisms are active. This can be demonstrated by viewing a white piece of paper under incandescent illumination and comparing the appearance to that of a CRT (or LCD) display of a uniform eld with exactly the same chromaticity and luminance viewed in a darkened room. The paper will appear white or just slightly yellowish. The display will appear relatively high-chroma yellow. In fact, a white piece of paper illuminated by that display will appear white while the display itself retains a yellow appearance! Color appearance models such as RLAB, the Hunt model, and CIECAM02 include provisions for various degrees of cognitive discounting-the-illuminant.

These standard deviations s (or the %RSD) are then plotted against the concentration LOD and LOQ values are those concentrations of analyte corresponding to %RSD values of 33 and 10%, respectively [15, 21] As is said for the LOD, neither is it true that at and below the LOQ quanti cation becomes impossible Quanti cation is possible; however it becomes unreliable as the uncertainty associated with it at these lower levels is higher than the measure-.

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